Porfirio B, Dallapiccola B, Cittanti C, Colamussi P, Giganti M, Piffanelli A
Department of Public Health, Tor Vergata University, Rome, Italy.
Acta Radiol. 1994 Jan;35(1):58-61.
An in vitro system has been set up to study the possible genetic effects of diagnostic ultrasound (US) on the developing fetus. Amniotic cells were cultured by the in situ technique. Well established clones were exposed to US for various lengths of time using a linear array transducer within a sound transparent apparatus. Cells were then grown in the presence of 5-bromo-2'-deoxyuridine and processed for scoring the number of sister chromatid exchanges (SCE) as the cytogenetic endpoint. There was no linear relationship between the SCE frequency and the duration of US exposure. Variance analysis showed that only interindividual variability was a significant component of total variation. Neither the main effect of treatment nor the interaction effect were statistically significant. The data suggest that US delivered from a diagnostic unit to actively growing cultured fetal cells in a system closely mimicking the conditions of US exposure during amniocentesis does not induce SCE.
已建立一个体外系统来研究诊断性超声（US）对发育中胎儿可能产生的遗传效应。采用原位技术培养羊膜细胞。使用线性阵列换能器在透声装置内，将成熟的克隆暴露于超声不同时长。然后让细胞在5-溴-2'-脱氧尿苷存在的情况下生长，并进行处理以计算姐妹染色单体交换（SCE）的数量作为细胞遗传学终点。SCE频率与超声暴露持续时间之间不存在线性关系。方差分析表明，个体间变异性是总变异的显著组成部分。治疗的主效应和交互效应均无统计学意义。数据表明，在一个紧密模拟羊膜穿刺术期间超声暴露条件的系统中，从诊断设备发出的超声不会诱导活跃生长的培养胎儿细胞发生SCE。
