Poinar G O
College of Natural Resources, University of California, Berkeley 94720.
Experientia. 1994 Jun 15;50(6):536-42. doi: 10.1007/BF01921722.
A survey of the major fossiliferous amber deposits is provided, including ages and various categories of life forms reported from each. The frequency of occurrence of the major groups of plants and animals in these amber deposits is also given. Thus far, DNA from four insect and one plant species has been extracted from amber fossils. In the case of the stingless bee in Dominican amber, evidence of reproducibility is provided, since two independent laboratories isolated DNA from six or more different specimens of the same insect. Amber sources for DNA studies are listed together with their advantages and disadvantages. The important points are the availability of desired pieces, the proper identification of the fossil, verification of the amber deposit, the cost involved, and the feasibility of causing damage to the specimen. The availability of several types of amber (Mexican, Dominican, Baltic, Chinese, Canadian, Siberian and Lebanese) at four major sources (academic collections, commercial dealers, private collections and amber mines) is discussed. The scientific implications of obtaining DNA from amber inclusions are presented.
本文对主要的含化石琥珀矿床进行了调查,包括各矿床的年代以及所报道的各类生命形式。文中还给出了这些琥珀矿床中主要动植物类群的出现频率。迄今为止,已从琥珀化石中提取出四种昆虫和一种植物的DNA。对于多米尼加琥珀中的无刺蜂,由于两个独立实验室从同一昆虫的六个或更多不同标本中分离出了DNA,因此提供了可重复性的证据。列出了用于DNA研究的琥珀来源及其优缺点。重点在于所需样本的可获取性、化石的正确鉴定、琥珀矿床的核实、相关成本以及对标本造成损坏的可行性。讨论了在四个主要来源(学术收藏、商业经销商、私人收藏和琥珀矿)获取几种类型琥珀(墨西哥琥珀、多米尼加琥珀、波罗的海琥珀、中国琥珀、加拿大琥珀、西伯利亚琥珀和黎巴嫩琥珀)的情况。阐述了从琥珀内含物中获取DNA的科学意义。