11号染色体q22.3区域共济失调毛细血管扩张症区段的脉冲场凝胶电泳图谱。
A pulsed-field gel electrophoresis map in the ataxia-telangiectasia region of chromosome 11q22.3.
作者信息
Uhrhammer N, Concannon P, Huo Y, Nakamura Y, Gatti R A
机构信息
Department of Pathology, School of Medicine, University of California, Los Angeles 90024.
出版信息
Genomics. 1994 Mar 15;20(2):278-80. doi: 10.1006/geno.1994.1165.
Our interest in isolating the gene(s) for ataxia-telangiectasia has prompted us to construct a physical map of chromosome 11q22.3 using markers localized to this region by linkage analysis and/or hybrid cell panels. Twenty-two markers have been analyzed by pulsed-field gel electrophoresis. Nine of these markers form an approximately 2-Mb long-range contiguous map. An average distance of 200 kb between probes in this map should facilitate the isolation of new cDNAs, anonymous probes, and YACs in an orderly way.
我们对分离共济失调毛细血管扩张症的基因感兴趣，这促使我们利用通过连锁分析和/或杂交细胞板定位到该区域的标记构建11号染色体q22.3的物理图谱。已经通过脉冲场凝胶电泳分析了22个标记。其中9个标记形成了一个大约2兆碱基的长程连续图谱。该图谱中探针之间平均200千碱基的距离应有助于按顺序分离新的cDNA、匿名探针和酵母人工染色体。
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