Blázquez M A, Stucka R, Feldmann H, Gancedo C
Instituto de Investigaciones Biomédicas, Consejo Superior de Investigaciones Científicas, Madrid, Spain.
J Bacteriol. 1994 Jul;176(13):3895-902. doi: 10.1128/jb.176.13.3895-3902.1994.
Trehalose-6-P inhibits hexokinases in Saccharomyces cerevisiae (M. A. Blázquez, R. Lagunas, C. Gancedo, and J. M. Gancedo, FEBS Lett. 329:51-54, 1993), and disruption of the TPS1 gene (formerly named CIF1 or FDP1) encoding trehalose-6-P synthase prevents growth in glucose. We have found that the hexokinase from Schizosaccharomyces pombe is not inhibited by trehalose-6-P even at a concentration of 3 mM. The highest internal concentration of trehalose-6-P that we measured in S. pombe was 0.75 mM after heat shock. We have isolated from S. pombe the tps1+ gene, which is homologous to the Saccharomyces cerevisiae TPS1 gene. The DNA sequence from tps1+ predicts a protein of 479 amino acids with 65% identity with the protein of S. cerevisiae. The tps1+ gene expressed from its own promoter could complement the lack of trehalose-6-P synthase in S. cerevisiae tps1 mutants. The TPS1 gene from S. cerevisiae could also restore trehalose synthesis in S. pombe tps1 mutants. A chromosomal disruption of the tps1+ gene in S. pombe did not have a noticeable effect on growth in glucose, in contrast with the disruption of TPS1 in S. cerevisiae. However, the disruption prevented germination of spores carrying it. The level of an RNA hybridizing with an internal probe of the tps1+ gene reached a maximum after 20 min of heat shock treatment. The results presented support the idea that trehalose-6-P plays a role in the control of glycolysis in S. cerevisiae but not in S. pombe and show that the trehalose pathway has different roles in the two yeast species.
海藻糖-6-磷酸抑制酿酒酵母中的己糖激酶（M. A. 布拉兹克斯、R. 拉古纳斯、C. 甘塞多和J. M. 甘塞多，《欧洲生物化学学会联合会快报》329:51 - 54，1993），编码海藻糖-6-磷酸合酶的TPS1基因（原名为CIF1或FDP1）的破坏会阻止在葡萄糖中的生长。我们发现粟酒裂殖酵母的己糖激酶即使在3 mM的浓度下也不会被海藻糖-6-磷酸抑制。我们在粟酒裂殖酵母中测量到的海藻糖-6-磷酸的最高内部浓度在热休克后为0.75 mM。我们从粟酒裂殖酵母中分离出了tps1⁺基因，它与酿酒酵母的TPS1基因同源。从其自身启动子表达的tps1⁺基因可以弥补酿酒酵母tps1突变体中海藻糖-6-磷酸合酶的缺乏。酿酒酵母的TPS1基因也可以恢复粟酒裂殖酵母tps1突变体中的海藻糖合成。与酿酒酵母中TPS1的破坏相反，粟酒裂殖酵母中tps1⁺基因的染色体破坏对在葡萄糖中的生长没有明显影响。然而，这种破坏阻止了携带它的孢子的萌发。与tps1⁺基因内部探针杂交的RNA水平在热休克处理20分钟后达到最大值。所呈现的结果支持了海藻糖-6-磷酸在酿酒酵母中参与糖酵解控制而在粟酒裂殖酵母中不参与的观点，并表明海藻糖途径在这两种酵母物种中具有不同的作用。
