嗜热酵母多形汉逊酵母在高温下生长不需要海藻糖合成，但正常获得耐热性则需要。
The thermophilic yeast Hansenula polymorpha does not require trehalose synthesis for growth at high temperatures but does for normal acquisition of thermotolerance.
作者信息
Reinders A, Romano I, Wiemken A, De Virgilio C
机构信息
Botanisches Institut der Universität, CH-4056 Basel, Switzerland.
出版信息
J Bacteriol. 1999 Aug;181(15):4665-8. doi: 10.1128/JB.181.15.4665-4668.1999.
Abstract
The TPS1 gene from Hansenula polymorpha, which encodes trehalose-6-phosphate (Tre6P) synthase, has been isolated and characterized. The deletion of TPS1 rendered H. polymorpha cells incapable of trehalose synthesis under conditions where wild-type cells normally accumulate high levels of trehalose. Interestingly, the loss of Tre6P synthase did not cause any obvious growth defects on a glucose-containing medium, even at high temperatures, but seriously compromised the cells' ability to acquire thermotolerance.
摘要
多形汉逊酵母的TPS1基因已被分离和鉴定，该基因编码海藻糖-6-磷酸（Tre6P）合酶。在野生型细胞通常积累高水平海藻糖的条件下，TPS1基因的缺失使多形汉逊酵母细胞无法合成海藻糖。有趣的是，Tre6P合酶的缺失在含葡萄糖的培养基上并未导致任何明显的生长缺陷，即使在高温下也是如此，但严重损害了细胞获得耐热性的能力。
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