用于裂殖酵母的硫胺素可抑制表达载体pREP和pRIP。
Thiamine-repressible expression vectors pREP and pRIP for fission yeast.
作者信息
Maundrell K
机构信息
Sclavo Research Centre, Siena, Italy.
出版信息
Gene. 1993 Jan 15;123(1):127-30. doi: 10.1016/0378-1119(93)90551-d.
PMID:8422996
Abstract
The promoter and polyadenylation signal of the thiamine-repressible gene nmt1 of Schizosaccharomyces pombe have been used to construct the pREP extrachromosomally replicating plasmids and the pRIP integrative expression plasmids. These plasmids permit thiamine-mediated control of transcription to be applied to cloned genes.
摘要
粟酒裂殖酵母硫胺素可抑制基因nmt1的启动子和聚腺苷酸化信号已被用于构建pREP染色体外复制质粒和pRIP整合表达质粒。这些质粒使硫胺素介导的转录控制能够应用于克隆基因。
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