Sandler S J, Clark A J
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
J Bacteriol. 1994 Jul;176(13):4011-6. doi: 10.1128/jb.176.13.4011-4016.1994.
The level of translation of recF-lacZ fusions is reduced 20-fold by nucleotides 49 to 146 of recF. In this region of recF, we found a previously described ribosome-interactive sequence called epsilon and a hexapyrimidine tract located just upstream of the epsilon sequence. Mutational studies indicate that the hexapyrimidine sequence is involved in at least some of the reduced translation. When the hexapyrimidine sequence is mutant, mutating epsilon increases the level of translation maximally. We ruled out the possibility that ribosome frameshifting explains most of the effect of these two sequences on expression and suspect that multiple mechanisms may be responsible. In a separate report, we show that mutations in the hexapyrimidine tract and epsilon increase expression of the full-sized recF gene.
recF基因49至146位核苷酸可使recF - lacZ融合蛋白的翻译水平降低20倍。在recF基因的这一区域，我们发现了一个先前描述的名为ε的核糖体相互作用序列以及位于ε序列上游的一个六嘧啶序列。突变研究表明，六嘧啶序列至少参与了部分翻译水平的降低。当六嘧啶序列发生突变时，ε序列的突变会使翻译水平最大程度地提高。我们排除了核糖体移码解释这两个序列对表达影响的可能性，并怀疑可能有多种机制在起作用。在另一篇报告中，我们表明六嘧啶序列和ε序列的突变会增加全长recF基因的表达。
