Szilágyi J F, Berriman J
MRC Virology Unit, University of Glasgow, U.K.
J Gen Virol. 1994 Jul;75 ( Pt 7):1749-53. doi: 10.1099/0022-1317-75-7-1749.
The fine structure of light (L) particles of herpes simplex virus type 1 was examined by cryo-electron microscopy and compared to that of virions. The L particles appeared to be spherical entities with significant variation in size, on average smaller in diameter than virions (140 nm compared to 180 nm). The technique confirmed that L particles are composed of an outer envelope, i.e. a bilaminar membrane with protruding glycoprotein spikes, and a uniformly granular tegument, but lack any nucleocapsid. In addition it revealed the presence of one or occasionally more spherical objects, termed inclusion vesicles (IVs), embedded in the tegument of a large proportion of L particles but not observed in virions, suggesting that presence of IVs is unique to the L particles. The IVs vary in size and appear to be composed of a bilaminar membrane without surface projections and filled with material of relatively low electron density, suggesting that the composition of IVs is distinct from that of the envelope and tegument of the L particles.
通过冷冻电子显微镜检查了1型单纯疱疹病毒轻（L）颗粒的精细结构，并与病毒体的结构进行了比较。L颗粒似乎是球形实体，大小差异显著，平均直径比病毒体小（分别为140nm和180nm）。该技术证实，L颗粒由一个外膜组成，即一个带有突出糖蛋白刺突的双分子层膜，以及一层均匀的颗粒状皮层，但没有任何核衣壳。此外，它还揭示了在大部分L颗粒的皮层中存在一个或偶尔更多的球形物体，称为包涵体囊泡（IVs），但在病毒体中未观察到，这表明IVs的存在是L颗粒所特有的。IVs大小各异，似乎由一个没有表面突起的双分子层膜组成，内部充满电子密度相对较低的物质，这表明IVs的组成与L颗粒的包膜和皮层不同。
