Santos N, Gouvea V, Timenetsky M C, Clark H F, Riepenhoff-Talty M, Garbarg-Chenon A
Division of Molecular Biological Research and Evaluation, Food and Drug Administration, Washington, D.C. 20204.
J Gen Virol. 1994 Jul;75 ( Pt 7):1775-80. doi: 10.1099/0022-1317-75-7-1775.
Rotavirus strains belonging to G types 1 to 4 and having a P3 genotype (M37-like VP4) were recovered from children with symptomatic and asymptomatic infections. Partial sequences of their VP4 genes were determined in an attempt to characterize these strains further. The genomic regions encoding VP8*, the connecting and putative fusion peptides and three other regions in VP5* were sequenced. The deduced amino acid sequences were compared with rotavirus strains belonging to different P genotypes that had been previously reported. High degrees of identity were found between the VP8* fragment of all human P3 strains (92.7 to 99.7%) suggesting that they belong to the same genotype, regardless of differences in their virulence. Furthermore, based on comparative sequence analysis, we did not identify any amino acid(s) that differ appreciably between symptomatic and asymptomatic strains and could therefore be associated with virulence. The results suggest that the P3 genotype, although frequently associated with asymptomatic infections, may not be the single determining factor in attenuation of symptoms.
从有症状和无症状感染的儿童中分离出属于G1至G4型且具有P3基因型（M37样VP4）的轮状病毒株。测定了它们VP4基因的部分序列，以进一步鉴定这些毒株。对编码VP8*、连接肽和假定融合肽的基因组区域以及VP5中的其他三个区域进行了测序。将推导的氨基酸序列与先前报道的属于不同P基因型的轮状病毒株进行比较。在所有人类P3毒株的VP8片段之间发现了高度的同一性（92.7%至99.7%），这表明它们属于同一基因型，而不论其毒力差异如何。此外，基于比较序列分析，我们没有发现有症状和无症状毒株之间有明显差异且因此可能与毒力相关的任何氨基酸。结果表明，P3基因型虽然经常与无症状感染相关，但可能不是症状减轻的唯一决定因素。
