Thieme T, Piacentini S, Davidson S, Steingart K
Epitope Inc, Beaverton, OR 97005.
JAMA. 1994 Jul 20;272(3):219-21.
To determine if oral fluid samples can be used to reliably assess protective blood levels of antibodies to measles, mumps, and rubella.
A comparison of matched serum and oral fluid samples from asymptomatic subjects in enzyme-linked immunosorbent assays for measles, mumps, and rubella antibodies. A longitudinal study compared matched serum and oral fluid samples from 11 subjects after measles-mumps-rubella vaccination.
Five US clinical sites with samples tested at the reporting laboratory.
A total of 157 asymptomatic subjects including 55 subjects younger than 18 years.
The presence of antibodies in oral fluid specimens correlated with that in serum with the following levels of sensitivity and specificity: measles, 97% and 100%, respectively; mumps, 94% and 94%, respectively; rubella, 98% and 98%, respectively. Longitudinal studies of subjects after vaccination showed similar seroconversion profiles in oral fluid and serum samples.
Protective blood levels of antibodies to measles, mumps, and rubella can be assessed by means of an oral fluid sample with good reliability.
确定口腔液样本是否可用于可靠评估麻疹、腮腺炎和风疹抗体的保护性血液水平。
在酶联免疫吸附测定中，对无症状受试者的配对血清和口腔液样本进行麻疹、腮腺炎和风疹抗体检测，并进行比较。一项纵向研究比较了11名受试者在接种麻疹-腮腺炎-风疹疫苗后的配对血清和口腔液样本。
美国五个临床地点，样本在报告实验室进行检测。
共有157名无症状受试者，其中55名年龄小于18岁。
口腔液标本中的抗体存在情况与血清中的抗体存在情况具有相关性，其敏感性和特异性水平如下：麻疹分别为97%和100%；腮腺炎分别为94%和94%；风疹分别为98%和98%。对接种疫苗后的受试者进行的纵向研究显示，口腔液和血清样本中的血清转化情况相似。
通过口腔液样本可以可靠地评估麻疹、腮腺炎和风疹抗体的保护性血液水平。
