Orita H, Fukasawa M, Hirooka S, Uchino H, Fukui K, Kohi M, Washio M, Suzuki T
Second Department of Surgery, Yamagata University School of Medicine, Japan.
Jpn Heart J. 1994 Mar;35(2):213-23. doi: 10.1536/ihj.35.213.
The purpose of the present study was to evaluate the functional and biochemical effects of serum concentrations on hypothermic preservation of cardiac myocytes. Myocytes were isolated from neonatal rat ventricles and cultured for 4 days, and then incubated for 24 hrs at 4 degrees C in media containing 0, 2, 5, 10, and 20% fetal calf serum (FCS). The myocytes were then cultured for an additional 24 hrs at 37 degrees C to evaluate the recovery of myocyte beating rate. The recovery ratio of myocyte beating rate after hypothermic incubation was 25.3 +/- 14.2 percent of control in the serum-free, 33.0% in 2% FCS, 30.7% in 5% FCS, 22.3% in 10% FCS, and 24.3% in 20% FCS groups. Serum-free, 10% and 20% FCS groups had lower recovery ratios compared to the other two groups. The release of CPK and LDH after hypothermic incubation were similar in the serum-free, 2% and 5% FCS groups, however, significant increases were observed for the 10% and 20% FCS groups. It is suggested that fetal calf serum at high concentrations (> 10%) might be injurious to immature myocardium under hypothermic preservation, and that the optimum concentration of FCS is two percent or less.
本研究的目的是评估血清浓度对心肌细胞低温保存的功能和生化影响。从新生大鼠心室分离出心肌细胞并培养4天，然后在含有0%、2%、5%、10%和20%胎牛血清（FCS）的培养基中于4℃孵育24小时。随后将心肌细胞在37℃再培养24小时，以评估心肌细胞搏动率的恢复情况。低温孵育后，无血清组心肌细胞搏动率的恢复率为对照组的25.3±14.2%，2% FCS组为33.0%，5% FCS组为30.7%，10% FCS组为22.3%，20% FCS组为24.3%。无血清组、10%和20% FCS组的恢复率低于其他两组。低温孵育后，无血清组、2%和5% FCS组的CPK和LDH释放情况相似，然而，10%和20% FCS组则显著增加。提示高浓度（>10%）胎牛血清在低温保存下可能对未成熟心肌有损伤作用，FCS的最佳浓度为2%或更低。
