Yokozawa T, Yasui T, Ishii S, Oura H
Research Institute for Wakan-Yaku, Toyama Medical and Pharmaceutical University, Japan.
Nihon Jinzo Gakkai Shi. 1994 Apr;36(4):317-21.
Variations in the level of albumin mRNA and the transcription rate of the albumin gene in rats with adenine-induced renal failure were compared with those in normal rats. A paired feeding schedule was employed to eliminate any nutritional differences between normal rats and rats with adenine-induced renal failure. The albumin mRNA level isolated from the liver became lower as the period of adenine administration lengthened. However, there was no difference in the transcription rate of the albumin gene between the two rat groups. These results suggest that a post-transcriptional process is responsible for the renal failure-induced repression of albumin synthesis. Furthermore, plasma glucagon levels in adenine-induced renal failure specimens were markedly higher than those in the normal group, whereas we found no difference in the plasma insulin level between normal and adenine-fed rats. These investigations provide evidence that the decrease in the level of albumin mRNA in renal failure may be partly related to the elevated level of glucagon.
将腺嘌呤诱导的肾衰竭大鼠的白蛋白mRNA水平和白蛋白基因的转录速率与正常大鼠进行比较。采用配对喂养方案以消除正常大鼠和腺嘌呤诱导的肾衰竭大鼠之间的任何营养差异。随着腺嘌呤给药时间延长，从肝脏分离的白蛋白mRNA水平降低。然而，两组大鼠之间白蛋白基因的转录速率没有差异。这些结果表明，转录后过程是肾衰竭诱导的白蛋白合成抑制的原因。此外，腺嘌呤诱导的肾衰竭标本中的血浆胰高血糖素水平明显高于正常组，而我们发现正常大鼠和喂食腺嘌呤的大鼠之间的血浆胰岛素水平没有差异。这些研究提供了证据，表明肾衰竭中白蛋白mRNA水平的降低可能部分与胰高血糖素水平升高有关。
