Hills B P, Wright K M
A.F.R.C. Institute of Food Research, Norwich Research Park, Colney, U.K.
J Theor Biol. 1994 May 7;168(1):31-41. doi: 10.1006/jtbi.1994.1085.
A general theory of spatially dependent bacterial growth in heterogeneous systems is developed by combining a structured-cell kinetic model of bacterial metabolism with reaction-diffusion equations describing the transport of nutrients in the growth medium. Rate constants in the theory are determined for Listeria monocytogenes by fitting the viable count batch growth data acquired in homogeneous, shake-flask cultures. The rate constants are then used to calculate the form of bacterial growth when it occurs as localized microcolonies in structured systems. The same theory is used to analyse the effects of transient variations in environmental variables.
通过将细菌代谢的结构化细胞动力学模型与描述生长培养基中营养物质传输的反应扩散方程相结合，建立了非均匀系统中空间依赖性细菌生长的一般理论。通过拟合在均匀摇瓶培养中获得的活菌计数分批生长数据，确定了该理论中单核细胞增生李斯特菌的速率常数。然后，利用这些速率常数计算细菌在结构化系统中以局部微菌落形式生长时的生长形式。同一理论用于分析环境变量瞬态变化的影响。
