Bartel B, Fink G R
Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, Cambridge 02142.
Proc Natl Acad Sci U S A. 1994 Jul 5;91(14):6649-53. doi: 10.1073/pnas.91.14.6649.
Nitrilases (nitrile aminohydrolase, EC 3.5.5.1) convert nitriles to carboxylic acids. We report the cloning, characterization, and expression patterns of four Arabidopsis thaliana nitrilase genes (NIT1-4), one of which was previously described [Bartling, D., Seedorf, M., Mithöfer, A. & Weiler, E. W. (1992) Eur. J. Biochem. 205, 417-424]. The nitrilase genes encode very similar proteins that hydrolyze indole-3-acetonitrile to the phytohormone indole-3-acetic acid in vitro, and three of the four genes are tandemly arranged on chromosome III. Northern analysis using gene-specific probes and analysis of transgenic plants containing promoter-reporter gene fusions indicate that the four genes are differentially regulated. NIT2 expression is specifically induced around lesions caused by bacterial pathogen infiltration. The sites of nitrilase expression may represent sites of auxin biosynthesis in A. thaliana.
腈水解酶（腈氨基水解酶，EC 3.5.5.1）可将腈类化合物转化为羧酸。我们报告了4个拟南芥腈水解酶基因（NIT1 - 4）的克隆、特性及表达模式，其中一个基因先前已有报道[Bartling, D., Seedorf, M., Mithöfer, A. & Weiler, E. W. (1992) Eur. J. Biochem. 205, 417 - 424]。这些腈水解酶基因编码非常相似的蛋白质，它们在体外可将吲哚 - 3 - 乙腈水解为植物激素吲哚 - 3 - 乙酸，并且这4个基因中的3个在第三条染色体上串联排列。使用基因特异性探针的Northern分析以及对含有启动子 - 报告基因融合体的转基因植物的分析表明这4个基因受到不同的调控。NIT2的表达在细菌病原体浸润引起的损伤部位特异性诱导。腈水解酶的表达位点可能代表了拟南芥中生长素生物合成的位点。
