Konieczny A, Ausubel F M
Department of Genetics, Harvard Medical School, Boston, MA 02115.
Plant J. 1993 Aug;4(2):403-10. doi: 10.1046/j.1365-313x.1993.04020403.x.
A set of mapping markers have been designed for Arabidopsis thaliana that correspond to DNA fragments amplified by the polymerase chain reaction (PCR). The ecotype of origin of these amplified fragments can be determined by cleavage with a restriction endonuclease. Specifically, 18 sets of PCR primers were synthesized, each of which amplifies a single mapped DNA sequence from the Columbia and Landsberg erecta ecotypes. Also identified was at least one restriction endonuclease for each of these PCR products that generates ecotype-specific digestion patterns. Using these co-dominant cleaved amplified polymorphic sequences (CAPS), an Arabidopsis gene can be unambiguously mapped to one of the 10 Arabidopsis chromosome arms in a single cross using a limited number of F2 progeny.
已经为拟南芥设计了一组对应于通过聚合酶链反应（PCR）扩增的DNA片段的定位标记。这些扩增片段的原始生态型可以通过用限制性内切酶切割来确定。具体而言，合成了18组PCR引物，每组引物从哥伦比亚生态型和直立型生态型中扩增出单个定位的DNA序列。还为这些PCR产物中的每一个鉴定了至少一种产生生态型特异性消化模式的限制性内切酶。使用这些共显性切割扩增多态性序列（CAPS），可以在单个杂交中使用有限数量的F2后代将拟南芥基因明确地定位到10个拟南芥染色体臂中的一个上。
