Kokina N N
Ontogenez. 1975;6(5):503-12.
When cultivating the nervous tissue of newborn rats, rabbit embryos and tadpoles (as whole fragments or dissociated cells), 3 groups of neuroblasts are distinguished: differentiating, migrating and with growing processes. These groups correspond to different stages of neuroblast development in vivo and their presence in the tissue cultures of different brain regions reflects the heterochrony of cell development in these regions. The neuroblasts realize their morphogenetic potential within several days of cultivation irrespective of the contact with the other cells but the subsequent differentiation (dendrite branching, nuclear clearance etc.) proceeds only in cells united in aggregates.
在培养新生大鼠、兔胚胎和蝌蚪的神经组织(作为完整片段或解离细胞)时,可区分出3组神经母细胞:分化中的、迁移中的和具有生长突起的。这些组对应于体内神经母细胞发育的不同阶段,它们在不同脑区的组织培养物中的存在反映了这些区域细胞发育的异时性。神经母细胞在培养的几天内就能实现其形态发生潜能,而与其他细胞的接触无关,但随后的分化(树突分支、核清除等)仅在聚集成团的细胞中进行。