Panderi I, Parissi-Poulou M
Department of Pharmacy, University of Athens, Greece.
Analyst. 1994 Apr;119(4):697-701. doi: 10.1039/an9941900697.
A second-derivative spectrophotometric method for the determination of naproxen in the absence or presence of its 6-desmethyl metabolite in human plasma is described. The method consists of direct extraction of the non-ionized form of the drug with pure diethyl ether and determination of the naproxen by measuring the peak amplitude (mm) in the second-order derivative spectrum at a wavelength of 328.2 nm. The efficiency of the extraction procedure expressed by the absolute recovery was 94.6 +/- 0.7% (mean +/- s) for the concentration range tested, and the limit of quantification attained according to the IUPAC definition was 2.42 mg l-1. The linear dynamic range for naproxen was 5.0-100.0 mg l-1, the correlation coefficient for the calibration graphs was excellent, r = 0.99993 (n = 6), the precision (Sr) was better than 4.58% and the accuracy was satisfactory (Er < 2.32%). The results obtained by the proposed method were in good agreement with those found by an HPLC method.
本文描述了一种二阶导数分光光度法，用于测定人血浆中不存在或存在萘普生6-去甲基代谢物时的萘普生含量。该方法包括用纯乙醚直接萃取药物的非离子化形式，并通过测量328.2 nm波长处二阶导数光谱中的峰高（mm）来测定萘普生。在所测试的浓度范围内，以绝对回收率表示的萃取过程效率为94.6±0.7%（平均值±标准差），根据国际纯粹与应用化学联合会（IUPAC）的定义，定量限为2.42 mg·l⁻¹。萘普生的线性动态范围为5.0 - 100.0 mg·l⁻¹，校准曲线的相关系数极佳，r = 0.99993（n = 6），精密度（Sr）优于4.58%，准确度令人满意（Er < 2.32%）。所提出方法获得的结果与高效液相色谱法（HPLC）得到的结果高度一致。
