Yamaji Y, Hata Y, Furuya Y, Yamadori I, Fujita J, Takahara J, Irino S
First Department of Internal Medicine, Kagawa Medical School, Japan.
Hum Cell. 1994 Mar;7(1):33-9.
A new human small cell lung carcinoma (SCLC) cell line, designated MT-428, was derived from a patient who showed neurological paraneoplastic syndrome (combined with subacute cerebellar degeneration and peripheral sensory neuropathy) and was established in tissue culture. This cell line exhibited small cell (variant type) morphology as observed by phase contrast and electron microscopy. The MT-428 cells had a doubling time of 72 hours. Chromosomal analysis showed complicated rearrangements at short and long chromosomes with a modal number of 68. Several tumor markers, NSE, TPA and CPK-BB, were detected in culture medium. This cell line had a cloning efficiency of 1.3% in 0.8% methylcellulose. Finally, it should be noticed that autoantibody against MT-428 cell was demonstrated in serum of the patient. We concluded that the MT-428 cell line may provide a suitable model for studies of neurological paraneoplastic syndrome.
一种新的人类小细胞肺癌（SCLC）细胞系，命名为MT - 428，源自一名患有神经副肿瘤综合征（合并亚急性小脑变性和周围感觉神经病变）的患者，并通过组织培养建立。通过相差显微镜和电子显微镜观察，该细胞系呈现小细胞（变异型）形态。MT - 428细胞的倍增时间为72小时。染色体分析显示短染色体和长染色体存在复杂重排，众数为68。在培养基中检测到几种肿瘤标志物，即神经元特异性烯醇化酶（NSE）、组织多肽抗原（TPA）和脑型肌酸磷酸激酶（CPK - BB）。该细胞系在0.8%甲基纤维素中的克隆效率为1.3%。最后，值得注意的是，在该患者的血清中检测到了针对MT - 428细胞的自身抗体。我们得出结论，MT - 428细胞系可能为神经副肿瘤综合征的研究提供一个合适的模型。
