Silva C L, Alves L M, Figueiredo F
Department of Parasitology, Microbiology and Immunology, School of Medicine of Ribeirão Preto, University of São Paulo, Brazil.
Microbiology (Reading). 1994 May;140 ( Pt 5):1189-94. doi: 10.1099/13500872-140-5-1189.
The role of cell wall polysaccharides in leucocyte recruitment and granuloma formation in paracoccidioidomycosis was investigated. The inflammatory cells recruitment to the peritoneal cavity in rats inoculated with cell wall fraction (CW-265 or F1-265) from an avirulent strain of Paracoccidioides brasiliensis (Pb265), was greater than that observed for the cell wall fraction (CW-HC or F1-HC) recovered from the virulent strain (PbHC). Moreover, the inoculation of F1-HC and F1-265 into the subcutaneous layer of mice resulted in the formation of nodular and not progressive granulomatous lesions. The size and mean time of evolution of these lesions was proportional to the degree of virulence of the sample from which they were derived. Analyses showed that both F1 fractions contained beta-glucan and chitin. Only beta-glucan was able to trigger attraction and concentric organization of polymorphonuclear neutrophils and macrophages at the inflammatory foci, and the difference in the concentration of this compound in the cell walls of PbHC and Pb265 could explain the inflammatory capacity exhibited by the two strains of P. brasiliensis.
研究了细胞壁多糖在副球孢子菌病中白细胞募集和肉芽肿形成中的作用。用来自巴西副球孢子菌无毒株（Pb265）的细胞壁组分（CW - 265或F1 - 265）接种大鼠后，炎症细胞向腹腔的募集比从有毒株（PbHC）回收的细胞壁组分（CW - HC或F1 - HC）观察到的更多。此外，将F1 - HC和F1 - 265接种到小鼠皮下层导致形成结节性且不进展的肉芽肿性病变。这些病变的大小和平均演变时间与它们所源自样本的毒力程度成正比。分析表明，两种F1组分均含有β - 葡聚糖和几丁质。只有β - 葡聚糖能够在炎症灶引发多形核中性粒细胞和巨噬细胞的吸引和同心组织，并且该化合物在PbHC和Pb265细胞壁中的浓度差异可以解释巴西副球孢子菌两株菌所表现出的炎症能力。
