Silva M F, Silva C L
Department of Parasitology, Microbiology and Immunology, University of São Paulo, Brazil.
Clin Exp Immunol. 1995 Aug;101(2):321-7. doi: 10.1111/j.1365-2249.1995.tb08358.x.
In this study, we report an increase of the number of antibody-secreting cells and the augmentation of antibody production against unrelated antigens in mice infected with the fungus P. brasiliensis, as well as in mice inoculated with cell wall preparation isolated from P. brasiliensis (CW). The immunomodulatory effect of the live fungus and the CW preparation was dose-dependent, and their actions were mainly restricted to the i.v. or i.p. inoculation simultaneously with the sheep erythrocyte challenge by the i.v. route or restricted to i.p. inoculation of CW when bovine serum albumin (BSA) antigen was used. The dependence of antibody production on different routes of CW inoculation was correlated with the number of antigen-specific B cells in the spleen as determined by direct and reverse plaque-forming cell assays. The immunization schedules using CW preparation caused a preferential production of IgM and IgG3 antibodies. The results also showed that the hyperactive humoral immune response of mice induced by i.p. inoculation of CW was devoid of polyclonal B cell activation compared with the effects observed for the lipopolysaccharide (LPS)-treated groups. Paracoccidioides brasiliensis CW components may have potent immunological properties related to the non-specific B cell activation found in paracoccidioidomycosis.
在本研究中，我们报告了感染巴西副球孢子菌的小鼠以及接种从巴西副球孢子菌分离的细胞壁制剂（CW）的小鼠中，抗体分泌细胞数量增加，以及针对无关抗原的抗体产生增强。活真菌和CW制剂的免疫调节作用呈剂量依赖性，其作用主要限于静脉内（i.v.）或腹腔内（i.p.）接种，同时通过静脉内途径用绵羊红细胞进行攻击，或者当使用牛血清白蛋白（BSA）抗原时限于腹腔内接种CW。抗体产生对CW不同接种途径的依赖性与通过直接和反向空斑形成细胞试验测定的脾脏中抗原特异性B细胞数量相关。使用CW制剂的免疫方案导致优先产生IgM和IgG3抗体。结果还表明，与脂多糖（LPS）处理组所观察到的效应相比，腹腔内接种CW诱导的小鼠高活性体液免疫反应缺乏多克隆B细胞激活。巴西副球孢子菌CW成分可能具有与在副球孢子菌病中发现的非特异性B细胞激活相关的强大免疫特性。
