Nakagawa T, Maeshima M, Nakamura K, Asahi T
Laboratory of Biochemistry, School of Agriculture, Nagoya University, Japan.
Plant Cell Physiol. 1993 Jun;34(4):621-6.
We have cloned the gene for subunit Vc of sweet potato cytochrome c oxidase using its cDNA as a probe. There is a single intron, located one nucleotide upstream of the start of the open reading frame. No typical TATA-box sequence is present in the region upstream of the site of initiation of transcription in the cloned genomic DNA, suggesting that an alternative sequence may function in control of the expression of this gene. The nuclear genome of sweet potato contains only a single species of gene for subunit Vc.
我们利用甘薯细胞色素c氧化酶亚基Vc的cDNA作为探针，克隆了该基因。该基因有一个单一内含子，位于开放阅读框起始位置上游一个核苷酸处。在克隆的基因组DNA中转录起始位点上游区域不存在典型的TATA盒序列，这表明可能有一个替代序列在该基因的表达调控中发挥作用。甘薯的核基因组仅包含一种亚基Vc基因。
