Yoshioka H, Goma H, Nioka S, Ochi M, Miyake H, Zaman A, Masumura M, Sawada T, Chance B
Department of Pediatrics, Kyoto Prefectural University of Medicine, Japan.
Brain Res Dev Brain Res. 1994 Apr 15;78(2):273-8. doi: 10.1016/0165-3806(94)90036-1.
At 14 days of age, seven mongrel puppies were anesthetized and bilateral carotid arteries, not only the common but also the external and internal carotid arteries, were ligated with sutures. Seven sham-operated littermates served as controls. They were sacrificed at 3 months of age, and their brains were examined macro- and microscopically. Neuropathological examination revealed dilated posterior communicating and basilar arteries in bilateral carotid artery occlusion (BCAO) animals. Six out of 7 BCAO brains had uni- or multiloculated cysts in the periventricular white matter which were surrounded by a band of GFAP-positive glial cells. Scattered small areas of gliosis were found in all experimental animals. Four BCAO brains also showed ventricular dilatation. Although the cerebral cortex seemed to be intact, the periventricular white matter and the corpus callosum were reduced in width in experimental animals. Myelination in the white matter was significantly reduced in BCAO animals compared with the controls. This study directly demonstrates that cerebral hypoperfusion alone can produce periventricular leukomalacia in neonatal dogs.
在14日龄时，对7只杂种幼犬实施麻醉，并用缝线结扎双侧颈动脉，不仅包括颈总动脉，还包括颈外动脉和颈内动脉。7只假手术的同窝幼犬作为对照。它们在3月龄时被处死，并对其大脑进行大体和显微镜检查。神经病理学检查显示，双侧颈动脉闭塞（BCAO）动物的后交通动脉和基底动脉扩张。7只BCAO大脑中有6只在脑室周围白质中有单房或多房囊肿，其周围有一圈GFAP阳性胶质细胞。在所有实验动物中均发现散在的小面积胶质增生。4只BCAO大脑还显示脑室扩张。尽管大脑皮层似乎完好无损，但实验动物的脑室周围白质和胼胝体宽度减小。与对照组相比，BCAO动物白质中的髓鞘形成明显减少。本研究直接表明，单纯脑灌注不足可导致新生犬脑室周围白质软化。
