Haas H, Budowle B, Weiler G
Institute of Legal Medicine, Justus-Liebig University, Giessen, Germany.
Electrophoresis. 1994 Feb;15(2):153-8. doi: 10.1002/elps.1150150126.
A discontinuous borate/formate buffer system is presented for horizontal polyacrylamide gel electrophoresis of DNA fragments. The resolution potential of the system could be altered by changing the total monomer concentration (5-9%T), the concentration of the crosslinker piperazine diacrylamide (2-5%CPDA), as well as the concentration of formate in the gel (40-120 mM), the leading ion of the buffer system. The separation of DNA fragments would be improved by increasing the migration distance from 22 to 28 cm. This discontinuous polyacrylamide gel electrophoresis system proved highly reproducible.
本文介绍了一种用于DNA片段水平聚丙烯酰胺凝胶电泳的不连续硼酸盐/甲酸盐缓冲系统。通过改变总单体浓度（5-9%T）、交联剂哌嗪二丙烯酰胺的浓度（2-5%CPDA）以及凝胶中甲酸盐（缓冲系统的前导离子）的浓度（40-120 mM），可以改变该系统的分辨率。将迁移距离从22厘米增加到28厘米可改善DNA片段的分离效果。这种不连续聚丙烯酰胺凝胶电泳系统具有高度的可重复性。
