Velázquez E, Cruz-Sánchez J M, Mateos P F, Martínez-Molina E
Departamento de Microbiología et Genetica, Universidad de Salamanca, Spain.
Appl Environ Microbiol. 1998 Apr;64(4):1555-9. doi: 10.1128/AEM.64.4.1555-1559.1998.
Staircase electrophoresis in polyacrylamide gels was used to analyze the stable low-molecular-weight (LMW) RNA profiles of 24 type strains belonging to the family Rhizobiaceae. This new electrophoretic technique results in good separation of the molecules forming the LMW RNA profiles. Differences in the number and distribution of the RNA bands in these profiles allowed us to identify differences among the 24 strains assayed. Species assignments based on LMW RNAs proved to be consistent with the established taxonomic classification. Analysis of the data obtained and the corresponding dendrograms revealed relationships between genera and species; these relationships were essentially the same as those obtained with other techniques, such as DNA hybridization and 16S rRNA sequencing. Use of the technique described here, with which it is possible to analyze a large number of strains in a short time, permits rapid identification of species belonging to the family Rhizobiaceae and should in the future facilitate biodiversity studies and detection of new species.
采用聚丙烯酰胺凝胶中的阶梯式电泳法分析了根瘤菌科24个模式菌株的稳定低分子量（LMW）RNA图谱。这种新的电泳技术能很好地分离构成LMW RNA图谱的分子。这些图谱中RNA条带数量和分布的差异使我们能够识别所检测的24个菌株之间的差异。基于LMW RNA的物种分类结果与既定的分类学分类一致。对所得数据和相应的树状图进行分析，揭示了属与种之间的关系；这些关系与通过其他技术（如DNA杂交和16S rRNA测序）获得的关系基本相同。使用本文所述的技术，可以在短时间内分析大量菌株，从而能够快速鉴定根瘤菌科的物种，并且在未来应有助于生物多样性研究和新物种的检测。
