Guttman A, Shieh P, Hoang D, Horváth J, Cooke N
Beckman Instruments, Inc., Fullerton, CA 92634.
Electrophoresis. 1994 Feb;15(2):221-4. doi: 10.1002/elps.1150150137.
Capillary sodium dodecyl sulfate (SDS)-gel electrophoresis, for fast and high-resolution separations of protein molecules based on their molecular masses was investigated, employing a polymeric sieving network of polyethylene oxide (PEO). A standard mixture of five proteins ranging from 14.2-66.0 kDa in molecular mass was used to evaluate the sieving matrix. A general migration velocity equation was derived for capillary SDS-gel electrophoresis of proteins and supported by the experimental data. This equation gives a better understanding of SDS-protein separations via capillary gel electrophoresis. Results are presented regarding the effects of different operational variables such as gel concentration, electric field strength, molecular mass, and temperature on the electrophoretic migration properties of the different size protein molecules.
研究了基于聚环氧乙烷（PEO）的聚合物筛分网络的毛细管十二烷基硫酸钠（SDS）凝胶电泳，用于基于蛋白质分子量快速、高分辨率地分离蛋白质分子。使用分子量范围为14.2 - 66.0 kDa的五种蛋白质的标准混合物来评估筛分基质。推导了蛋白质毛细管SDS凝胶电泳的一般迁移速度方程，并得到了实验数据的支持。该方程有助于更好地理解通过毛细管凝胶电泳进行的SDS - 蛋白质分离。给出了关于不同操作变量（如凝胶浓度、电场强度、分子量和温度）对不同大小蛋白质分子电泳迁移特性影响的结果。
