Guttman A, Cooke N, Starr C M
Beckman Instruments, Inc., Fullerton, CA 9222634.
Electrophoresis. 1994 Dec;15(12):1518-22. doi: 10.1002/elps.11501501217.
The influence of operating variables on the separation of labeled oligosaccharide molecules in capillary electrophoresis with polymer networks is presented. In this study, an equation was derived that relates the effects of electrophoresis variables such as field strength, temperature, molecular weight and gel concentration to the migration velocity. As a model system, 8-aminonaphthalene-1,3,6-trisulfonate ANTS-labeled wheat starch digest was examined to show the validity of the equation. As an illustration of the high resolving power achievable with capillary electrophoresis, oligosaccharide molecules up to the degree of polymerization of 25 were separated with the very high efficiency of N > 10(6) and efficiency generation rate of 3500 plates/m/s for maltose. Separation based on the size of the oligosaccharides was not obtained with either entangled or unentangled polymer solutions.
本文介绍了操作变量对聚合物网络毛细管电泳中标记寡糖分子分离的影响。在本研究中，推导了一个方程，该方程将电场强度、温度、分子量和凝胶浓度等电泳变量的影响与迁移速度联系起来。作为模型系统，对8-氨基萘-1,3,6-三磺酸（ANTS）标记的小麦淀粉消化物进行了研究，以证明该方程的有效性。作为毛细管电泳可实现高分辨率的一个例证，聚合度高达25的寡糖分子得以分离，麦芽糖的分离效率非常高，N>10(6)，效率生成速率为3500板/米/秒。无论是缠结还是未缠结的聚合物溶液，都无法基于寡糖大小进行分离。
