Giri J G, Ahdieh M, Eisenman J, Shanebeck K, Grabstein K, Kumaki S, Namen A, Park L S, Cosman D, Anderson D
Immunex Research and Development Corporation, Seattle, WA 98101.
EMBO J. 1994 Jun 15;13(12):2822-30. doi: 10.1002/j.1460-2075.1994.tb06576.x.
We have recently cloned a novel cytokine, IL-15, with shared bioactivities but no sequence homology with IL-2. We found high affinity IL-15 binding to many cell types, including cells of non-lymphoid origin. Analysis of IL-15 interaction with subunits of the IL-2 receptor (IL-2R) revealed that the alpha subunit was not involved in IL-15 binding. We demonstrated directly in cells transfected with IL-2R subunits that both the beta and gamma chains are required for IL-15 binding and signaling. Hence, IL-15, like IL-2, IL-4 and IL-7, utilizes the common IL-2R gamma subunit found to be defective in X-linked severe combined immunodeficiency in humans. IL-15 is the only cytokine other than IL-2 that has also been shown to share the beta signaling subunit of IL-2R. The differential ability of some cells to bind and respond to IL-2 and IL-15 implies the existence of an additional IL-15-specific component.
我们最近克隆了一种新型细胞因子IL-15，它具有与IL-2相同的生物活性，但与IL-2没有序列同源性。我们发现IL-15能与多种细胞类型高亲和力结合，包括非淋巴细胞来源的细胞。对IL-15与IL-2受体（IL-2R）亚基相互作用的分析表明，α亚基不参与IL-15的结合。我们在转染了IL-2R亚基的细胞中直接证明，β链和γ链都是IL-15结合和信号传导所必需的。因此，IL-15与IL-2、IL-4和IL-7一样，利用了在人类X连锁严重联合免疫缺陷中发现有缺陷的常见IL-2Rγ亚基。IL-15是除IL-2之外唯一一种也被证明共用IL-2Rβ信号亚基的细胞因子。一些细胞结合并对IL-2和IL-15作出反应的能力差异意味着存在一种额外的IL-15特异性成分。
