Edom F, Mouly V, Barbet J P, Fiszman M Y, Butler-Browne G S
URA CNRS 1448, UFR Biomédicale des Sts-Pères, Paris, France.
Dev Biol. 1994 Jul;164(1):219-29. doi: 10.1006/dbio.1994.1193.
In order to test the diversification among satellite cells in man, satellite cells were isolated from human quadriceps and masseter muscles. The growth kinetics and morphological features of these cells were determined in vitro and the expression of the different myosin heavy (embryonic, fetal, fast, and slow) and light chain isoforms was analyzed. In all satellite cell cultures, only the four fast-type light chains (MLC1emb, MLC1F, MLC2F, and MLC3F) were synthesized and no slow myosin light chains were ever detected. However, we found that fused cultures of human satellite cells express both adult fast and slow myosin heavy chains (MHCs), in addition to embryonic and fetal isoforms. In order to determine if distinct fast and slow cell lineages could be detected among the satellite cells, a clonal analysis was carried out on both cell populations. This analysis was first carried out on clonal populations and was confirmed by the analysis of isolated clones. Double-labeling experiments confirmed that all myogenic clones which expressed fast MHC also coexpressed slow MHC. Therefore, we found no evidence for the existence of different fast and slow satellite cell lineages in human postnatal skeletal muscle.
为了测试人类卫星细胞之间的多样性，从人类股四头肌和咬肌中分离出卫星细胞。在体外测定这些细胞的生长动力学和形态特征，并分析不同肌球蛋白重链（胚胎型、胎儿型、快肌型和慢肌型）和轻链亚型的表达。在所有卫星细胞培养物中，仅合成了四种快肌型轻链（MLC1emb、MLC1F、MLC2F和MLC3F），从未检测到慢肌球蛋白轻链。然而，我们发现人类卫星细胞的融合培养物除了表达胚胎型和胎儿型亚型外，还表达成年快肌型和慢肌型肌球蛋白重链（MHCs）。为了确定在卫星细胞中是否能检测到不同的快肌型和慢肌型细胞谱系，对这两种细胞群体进行了克隆分析。该分析首先在克隆群体上进行，并通过对分离克隆的分析得到证实。双标记实验证实，所有表达快肌型MHC的成肌克隆也共表达慢肌型MHC。因此，我们没有发现证据表明人类出生后骨骼肌中存在不同的快肌型和慢肌型卫星细胞谱系。
