Spitzer S G, Spitzer E D
Department of Pathology, SUNY at Stony Brook 11794-8691.
Gene. 1994 Jun 24;144(1):103-6. doi: 10.1016/0378-1119(94)90211-9.
The pathogenic yeast Cryptococcus neoformans contains 10-20 dispersed repetitive elements that hybridize to clone CNRE-1.0. Screening of a genomic library with probes derived from CNRE-1.0 identified five phages with restriction maps that overlapped CNRE-1.0 and three additional phages that belonged to two distinct groups. Sequencing of internal 3.5-kb SstI fragments from two CNRE-1-like elements revealed 95% homology, as well as a conserved open reading frame. A PCR-RFLP assay was developed that can distinguish different subfamilies of CNRE-1-like elements.
