Lazarides E, Burridge K
Cell. 1975 Nov;6(3):289-98. doi: 10.1016/0092-8674(75)90180-4.
Antibodies specific for the skeletal muscle structural protein alpha-actinin are used to localize this protein by indirect immunofluorescence in nonmuscle cells. In cultured nonmuscle cells, alpha-actinin is localized along or between actin filament bundles producing an almost regular periodicity. The protein is also detected in the form of fluorescent plaques at some ends of actin filament bundles, as well as in a filamentous form in some overlap areas of cells. In spreading rat embryo cells, alpha-actinin assumes a focal distribution which corresponds to the vertices of a highly regular actin filament network. The results suggest that alpha-actinin may be involved in the organization of actin filament bundles, in the attachment of actin filaments to the plasma membrane, and in the assembly of actin filaments in areas of cell to cell contact.
针对骨骼肌结构蛋白α-辅肌动蛋白的特异性抗体，被用于通过间接免疫荧光法在非肌肉细胞中定位该蛋白。在培养的非肌肉细胞中，α-辅肌动蛋白定位于肌动蛋白丝束之间或沿其分布，呈现出几乎规则的周期性。在肌动蛋白丝束的某些末端也能检测到呈荧光斑块形式的该蛋白，以及在细胞的一些重叠区域呈丝状形式存在。在铺展的大鼠胚胎细胞中，α-辅肌动蛋白呈现出局部分布，这与高度规则的肌动蛋白丝网络的顶点相对应。结果表明，α-辅肌动蛋白可能参与肌动蛋白丝束的组织、肌动蛋白丝与质膜的附着以及细胞间接触区域肌动蛋白丝的组装。
