Pérez-Serrano J, Casado N, Rodriguez-Caabeiro F
Laboratory of Parasitology, Faculty of Pharmacy, University of Alcalá de Henares, Madrid, Spain.
Int J Parasitol. 1994 Apr;24(2):219-24. doi: 10.1016/0020-7519(94)90029-9.
Protoscoleces of Echinococcus granulosus were incubated in vitro with decreasing concentrations of either albendazole (ABZ) or albendazole sulphoxide (ABZ.SO) (50, 10, 1 and 0.1 micrograms ml-1), and in combination. Viability was assessed by the methylene blue exclusion test and establishment of infection in mice. Protoscolex ultrastructure was determined by means of transmission and scanning electron microscopy. ABZ and ABZ.SO, when used separately had protoscolicidal activity after a longer incubation period (30 days) than when used as combined compounds. When incubated in the presence of ABZ + ABZ.SO, protoscolex viability dropped rapidly. That is, protoscoleces were all non-viable at 12 days of exposure, with no cyst developing following their inoculation into mice. The ultrastructural changes induced by ABZ or ABZ.SO alone, were: (a) rostellar disorganization, (b) formation of numerous blebs on the tegument, (c) loss of the microtriches, (d) increased vesiculation within the tegumentary cytons together, (e) an increase in lipid deposits and (f) depletion of glycogen reserves. After incubation with combined ABZ and ABZ.SO the tegument contained numerous blebs which became detached, leaving debris only, some intact nuclei being discernible in the protoscolex parenchyma.
将细粒棘球绦虫的原头节在体外与浓度逐渐降低的阿苯达唑（ABZ）或阿苯达唑亚砜（ABZ.SO）（50、10、1和0.1微克/毫升）单独及联合孵育。通过亚甲蓝排斥试验和小鼠感染确立情况评估活力。借助透射电子显微镜和扫描电子显微镜确定原头节超微结构。ABZ和ABZ.SO单独使用时，与联合使用相比，在较长孵育期（30天）后具有杀原头节活性。当在ABZ + ABZ.SO存在下孵育时，原头节活力迅速下降。也就是说，在暴露12天时原头节全部失去活力，接种到小鼠体内后无囊肿形成。单独使用ABZ或ABZ.SO诱导的超微结构变化包括：（a）顶突结构紊乱，（b）体表形成大量泡状突起，（c）微毛缺失，（d）体表细胞内空泡化增加，（e）脂质沉积增加，（f）糖原储备耗尽。与ABZ和ABZ.SO联合孵育后，体表含有大量脱落的泡状突起，仅留下碎片，在原头节实质中可辨认出一些完整的细胞核。
