Leon S P, Carroll R S, Dashner K, Glowacka D, Black P M
Neurosurgical Laboratories, Brigham and Women's Hospital, Boston, Massachusetts 02115.
J Clin Endocrinol Metab. 1994 Jul;79(1):51-5. doi: 10.1210/jcem.79.1.8027252.
Little is known about the expression of growth factors and their receptors in pituitary tumors or the relationship of growth factors to pituitary neoplasia. Platelet-derived growth factor (PDGF) is a potent mitogen that has been postulated to stimulate tumor growth through autocrine and/or paracrine loops in a number of human tumors. In the present study we demonstrate messenger ribonucleic acid expression of the PDGF subunits and receptors in a variety of human pituitary adenomas and in a normal human anterior pituitary gland. Northern blot analysis performed on 34 pituitary adenomas showed that all tumors expressed the PDGF-A and PDGF-B subunits, the majority (94%) expressed the PDGF-beta receptor, and a subset (44%) expressed the PDGF-alpha receptor. The normal anterior pituitary studied expressed all of the PDGF subunits and receptor subunits. Clinically, there was no correlation between expression of the PDGF subunits or receptors and tumor size or with invasion into adjacent structures (cavernous sinus, sphenoid sinus, or clivus). A higher proportion of the endocrinologically active adenomas expressed the PDGF-alpha receptor (5 of 8) compared to the endocrine inactive tumors (10 of 26). The function of PDGF in the pituitary is currently not known. The finding that PDGF subunit and receptor messenger ribonucleic acids are coexpressed in pituitary adenomas and normal anterior pituitary suggests that autocrine and/or paracrine loops involving PDGF may exist in pituitary adenomas and normal anterior pituitary.
关于生长因子及其受体在垂体肿瘤中的表达，以及生长因子与垂体肿瘤形成之间的关系，目前所知甚少。血小板衍生生长因子（PDGF）是一种强效的有丝分裂原，据推测它可通过自分泌和/或旁分泌途径刺激多种人类肿瘤的生长。在本研究中，我们证实了PDGF亚基及其受体在多种人类垂体腺瘤和正常人类垂体前叶中的信使核糖核酸表达。对34例垂体腺瘤进行的Northern印迹分析表明，所有肿瘤均表达PDGF - A和PDGF - B亚基，大多数（94%）表达PDGF - β受体，一小部分（44%）表达PDGF - α受体。所研究的正常垂体前叶表达所有的PDGF亚基和受体亚基。临床上，PDGF亚基或受体的表达与肿瘤大小或侵袭相邻结构（海绵窦、蝶窦或斜坡）之间无相关性。与无内分泌活性的肿瘤（26例中有10例）相比，有内分泌活性的腺瘤中表达PDGF - α受体的比例更高（8例中有5例）。目前尚不清楚PDGF在垂体中的功能。PDGF亚基和受体信使核糖核酸在垂体腺瘤和正常垂体前叶中共同表达这一发现表明，垂体腺瘤和正常垂体前叶中可能存在涉及PDGF的自分泌和/或旁分泌途径。
