Di Rocco F, Carroll R S, Zhang J, Black P M
Neurosurgical Laboratories, Brigham and Women's Hospital, Harvard Medical School, Boston, Massachusetts 02115, USA.
Neurosurgery. 1998 Feb;42(2):341-6. doi: 10.1097/00006123-199802000-00080.
Platelet-derived growth factor (PDGF) induces cellular proliferation and differentiation by activating intracellular signaling mechanisms via their cognate receptors. In previous studies, we demonstrated that human brain tumors such as meningiomas, astrocytomas, medulloblastomas, and ependymomas expressed the messenger ribonucleic acid for the PDGF subunits and their receptors. In the present study, we investigated the expression of the messenger ribonucleic acid PDGF A and B chains and the PDGF alpha and beta receptors in 17 cases of oligodendrogliomas.
Measurements of messenger ribonucleic acid levels were obtained using radioactive complementary deoxyribonucleic acid probes. Protein expression was analyzed with specific antibodies.
Sixteen of 17 tumors expressed the PDGF A subunit and all the PDGF alpha receptors. Furthermore, all the tumors expressed PDGF B and PDGF beta receptor subunits.
The results of this study suggest that oligodendrogliomas may have an autocrine loop stimulated by the interaction of PDGF and its receptor simultaneously produced by these tumors.
血小板衍生生长因子（PDGF）通过其同源受体激活细胞内信号传导机制来诱导细胞增殖和分化。在先前的研究中，我们证明人类脑肿瘤如脑膜瘤、星形细胞瘤、髓母细胞瘤和室管膜瘤表达PDGF亚基及其受体的信使核糖核酸。在本研究中，我们调查了17例少突胶质细胞瘤中信使核糖核酸PDGF A和B链以及PDGFα和β受体的表达情况。
使用放射性互补脱氧核糖核酸探针测量信使核糖核酸水平。用特异性抗体分析蛋白质表达。
17个肿瘤中有16个表达PDGF A亚基和所有的PDGFα受体。此外，所有肿瘤均表达PDGF B和PDGFβ受体亚基。
本研究结果表明，少突胶质细胞瘤可能存在一个由这些肿瘤同时产生的PDGF及其受体相互作用所刺激的自分泌环。
