使用鼻咽拭子的海藻酸钙纤维和铝杆组件对基于PCR的百日咳博德特氏菌检测法的抑制作用。
Inhibition of PCR-based assay for Bordetella pertussis by using calcium alginate fiber and aluminum shaft components of a nasopharyngeal swab.
作者信息
Wadowsky R M, Laus S, Libert T, States S J, Ehrlich G D
机构信息
Department of Pathology, University of Pittsburgh, Pennsylvania 15261.
出版信息
J Clin Microbiol. 1994 Apr;32(4):1054-7. doi: 10.1128/jcm.32.4.1054-1057.1994.
A PCR-based assay for Bordetella pertussis was inhibited by using a calcium alginate fiber-tipped swab with an aluminum shaft but not by using a Dacron fiber-tipped swab with a plastic shaft. The calcium alginate fiber component inhibited the assay following storage for less than 1 min in a suspension of 10(3) CFU of B. pertussis per ml, whereas the aluminum shaft component required storage for at least 48 h in order to cause inhibition. We recommend the Dacron swab over the calcium alginate swab for collecting specimens for testing in PCR-based assays.
用于百日咳博德特氏菌的基于聚合酶链反应（PCR）的检测方法，使用带有铝杆的海藻酸钙纤维头拭子会受到抑制，但使用带有塑料杆的涤纶纤维头拭子则不会。海藻酸钙纤维成分在每毫升含10³CFU百日咳博德特氏菌的悬液中储存不到1分钟后就会抑制该检测方法，而铝杆成分则需要储存至少48小时才会产生抑制作用。我们建议在基于PCR的检测中，采集用于检测的标本时使用涤纶拭子而非海藻酸钙拭子。
Zotero 插件