Cloud Joann L, Hymas Weston, Carroll Karen C
Associated Regional and University Pathologists (ARUP) Institute for Clinical and Experimental Pathology, 500 Chipeta Way, Salt Lake City, UT 84108, USA.
J Clin Microbiol. 2002 Oct;40(10):3838-40. doi: 10.1128/JCM.40.10.3838-3840.2002.
A study was designed to assess the performance of various swabs and transport media routinely used to collect specimens submitted for Bordetella culture and PCR. Calcium-alginate swabs inhibited the PCR. No inhibition was detected in any PCRs with dacron or rayon swabs. All swab materials performed similarly for recovery of Bordetella pertussis in culture. The Amies with charcoal transport system performed poorly for culture. Calcium-alginate swabs are not recommended for PCR-based detection of B. pertussis. Dacron and rayon swabs are an excellent choice for both PCR and culture.
一项研究旨在评估常用于收集提交进行博德特氏菌培养和PCR检测的标本的各种拭子和运送培养基的性能。藻酸钙拭子会抑制PCR。使用涤纶或人造纤维拭子进行的任何PCR检测均未检测到抑制作用。在培养中,所有拭子材料在百日咳博德特氏菌的回收方面表现相似。含活性炭的阿氏运送培养基在培养方面表现不佳。不建议使用藻酸钙拭子进行基于PCR的百日咳博德特氏菌检测。涤纶和人造纤维拭子是PCR和培养的绝佳选择。