Foreyt W J, Snipes K P, Kasten R W
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164.
J Wildl Dis. 1994 Apr;30(2):137-45. doi: 10.7589/0090-3558-30.2.137.
In a series of three experiments, isolates of Pasteurella haemolytica biotype A, serotype 2, ribotype reference WSU-1, from healthy domestic sheep, were inoculated intratracheally into eight bighorn sheep (Ovis canadensis canadensis) and seven domestic sheep with doses of bacteria ranging from 5.3 x 10(8) to 8.6 x 10(11) colony forming units. Seven of eight inoculated bighorn sheep died from acute pneumonia within 48 hr of inoculation, whereas all seven domestic sheep inoculated with comparable or greater doses of bacteria remained healthy. One contact control bighorn sheep also died 6 days after its penmates received P. haemolytica. Three other noncontact control bighorn sheep remained healthy during the experiments. Pasteurella haemolytica biotype A, serotype 2, ribotype reference WSU-1 in the inocula was recovered from one or more tissues from all bighorns that died; whereas, it was not detected in any bighorn sheep before inoculation. Three different ribotypes of P. haemolytica A2 were recovered from bighorn sheep; however, only the ribotype reference WSU-1 in the domestic sheep-origin inoculum was recovered from all dead bighorn sheep, and was not recovered from bighorn sheep that survived the experiments. Thus, a relatively nonpathogenic and common isolate of P. haemolytica from healthy domestic sheep was lethal in bighorn sheep under experimental conditions.
在一系列三个实验中，从健康家养绵羊分离出的溶血巴斯德氏菌生物型A、血清型2、核糖体分型参考株WSU - 1，经气管内接种到8只大角羊（加拿大盘羊指名亚种）和7只家养绵羊体内，接种细菌剂量范围为5.3×10⁸至8.6×10¹¹菌落形成单位。8只接种的大角羊中有7只在接种后48小时内死于急性肺炎，而接种了相当剂量或更高剂量细菌的7只家养绵羊均保持健康。一只接触对照组大角羊在其同圈伙伴接种溶血巴斯德氏菌6天后也死亡。另外三只非接触对照组大角羊在实验期间保持健康。接种物中的溶血巴斯德氏菌生物型A、血清型2、核糖体分型参考株WSU - 1从所有死亡大角羊的一个或多个组织中分离出来；而在接种前任何大角羊中均未检测到。从大角羊中分离出三种不同核糖体分型的溶血巴斯德氏菌A2；然而，只有来自家养绵羊源接种物中的核糖体分型参考株WSU - 1从所有死亡大角羊中分离出来，并且在实验中存活的大角羊中未分离到。因此，在实验条件下，从健康家养绵羊分离出的相对无致病性且常见的溶血巴斯德氏菌菌株对大角羊具有致死性。
