Silflow R M, Foreyt W J
Department of Veterinary Microbiology and Pathology, Washington State University, Pullman 99164.
J Wildl Dis. 1994 Oct;30(4):529-35. doi: 10.7589/0090-3558-30.4.529.
Alveolar macrophages and peripheral blood neutrophils from elk (Cervus elaphus), bighorn sheep (Ovis canadensis canadensis), and domestic sheep were exposed to culture supernatants from Pasteurella haemolytica isolated from bighorn sheep and domestic sheep. In a second experiment, peripheral blood neutrophils from mule deer (Odocoileus hemionus), elk, and bighorn sheep were exposed to culture supernatants from P. haemolytica isolated from elk, bighorn sheep and domestic sheep. Alveolar macrophages from elk, bighorn sheep and domestic sheep were resistant to killing by P. haemolytica supernatants from bighorn sheep and domestic sheep; susceptibility of neutrophils to cell death, as measured by release of lactate dehydrogenase, differed significantly (P < 0.05) between the four species tested. Bighorn sheep and domestic sheep neutrophils were susceptible to cytotoxin damage by the P. haemolytica isolates used; bighorn sheep neutrophils were four- to eight-fold more susceptible to cytotoxin damage than domestic sheep neutrophils. Neutrophils from deer and elk were resistant to killing by P. haemolytica cytotoxins from any species tested.
将来自驼鹿（马鹿）、大角羊（加拿大盘羊指名亚种）和家羊的肺泡巨噬细胞及外周血中性粒细胞暴露于从大角羊和家羊分离出的溶血巴斯德氏菌的培养上清液中。在第二个实验中，将来自骡鹿（白尾鹿）、驼鹿和大角羊的外周血中性粒细胞暴露于从驼鹿、大角羊和家羊分离出的溶血巴斯德氏菌的培养上清液中。来自驼鹿、大角羊和家羊的肺泡巨噬细胞对来自大角羊和家羊的溶血巴斯德氏菌上清液的杀伤具有抗性；在所测试的四个物种之间，中性粒细胞对细胞死亡的易感性（通过乳酸脱氢酶的释放来衡量）存在显著差异（P < 0.05）。大角羊和家羊的中性粒细胞对所使用的溶血巴斯德氏菌分离株的细胞毒素损伤敏感；大角羊的中性粒细胞对细胞毒素损伤的敏感性是家羊中性粒细胞的四至八倍。来自鹿和驼鹿的中性粒细胞对所测试的任何物种的溶血巴斯德氏菌细胞毒素的杀伤具有抗性。
