Kimura I, Nakashima N, Komori T, Kameda Y, Kimura M
Department of Chemical Pharmacology, Faculty of Pharmaceutical Sciences, Toyama Medical and Pharmaceutical University, Japan.
Jpn J Pharmacol. 1994 Feb;64(2):103-7. doi: 10.1254/jjp.64.103.
The glucose-dependency of cholecystokinin-8 (CCK-8)-stimulated insulin release was investigated at high (11.1 and 16.7 mM) glucose concentrations in rat pancreas perfusion and islet perifusion using glucose analogues, pseudo-alpha-D-glucose and 2-deoxy-D-glucose. In perfused pancreas, both glucose analogues (22.4 mM) inhibited high glucose (16.7 mM)-induced insulin release, but not normal glucose (5.6 mM)-induced insulin release, with or without CCK-8 (1 nM). In perifused islets, the same level of either of the glucose analogues inhibited high glucose (11.1 mM)-induced insulin release, with or without CCK-8 (100 nM). These results demonstrate that CCK-8-stimulated insulin release only at high glucose level is glucose-dependent.
