大麦黄矮病毒通读蛋白的检测

Detection of the readthrough protein of barley yellow dwarf virus.

作者信息

Cheng S L, Domier L L, D'Arcy C J

机构信息

Department of Plant Pathology, University of Illinois at Urbana-Champaign 61801.

出版信息

Virology. 1994 Aug 1;202(2):1003-6. doi: 10.1006/viro.1994.1427.

DOI:10.1006/viro.1994.1427

PMID:8030200

Abstract

The single open reading frame (ORF) 5 encoding the 50-kDa protein of barley yellow dwarf virus PAV-IL (BYDV-PAV-IL) was expressed in bacteria, purified, and used as an immunogen/antigen to produce/screen antibodies specific to the 50-kDa protein. Two monoclonal antibodies (MAb PAV-IL-22 kDa and MAb PAV-IL-50 kDa) raised against BYDV-PAV-IL could specifically detect the presence of the 72-kDa readthrough protein in extracts from the BYDV-infected leaf tissue. The results suggest that ORF 5 (50-kDa protein) is translated by readthrough of ORF 3 (22-kDa coat protein) to produce the 72-kDa protein. The readthrough protein is thought to be a structural protein on the external surface of BYDV.

摘要

编码大麦黄矮病毒PAV-IL（BYDV-PAV-IL）50 kDa蛋白的单一开放阅读框（ORF）5在细菌中表达、纯化，并用作免疫原/抗原来制备/筛选针对该50 kDa蛋白的特异性抗体。针对BYDV-PAV-IL产生的两种单克隆抗体（MAb PAV-IL-22 kDa和MAb PAV-IL-50 kDa）能够特异性检测BYDV感染的叶片组织提取物中72 kDa通读蛋白的存在。结果表明，ORF 5（50 kDa蛋白）通过ORF 3（22 kDa外壳蛋白）的通读进行翻译，从而产生72 kDa蛋白。通读蛋白被认为是BYDV外表面的一种结构蛋白。