Terhune P G, Heffess C S, Longnecker D S
Department of Pathology, Dartmouth Medical School, Lebanon, New Hampshire 037563.
Mol Carcinog. 1994 Jun;10(2):110-4. doi: 10.1002/mc.2940100209.
Activation of the c-Ki-ras proto-oncogene is more common in carcinomas of the pancreas than in other human carcinomas. Most such carcinomas are of the ductal cell phenotype. Ductal adenocarcinomas of the hamster pancreas have similar mutations, but acinar cell carcinomas of the mouse and rat pancreas lack the common c-Ki-ras mutations. Therefore, we examined 11 acinar cell carcinomas of the human pancreas for evidence of mutations at codons 12, 13, and 61. DNA was isolated from tumor cells in paraffin-embedded sections. The polymerase chain reaction was used to amplify the appropriate DNA sequence, and then allele-specific oligonucleotide hybridization and DNA sequence analysis were used to evaluate c-Ki-ras structure. Only wild-type sequences were found in codons 12, 13, and 61. Thus, in both the human and rodent species, mutations of c-Ki-ras appear to be more important in the genesis of ductal carcinomas than in the genesis of acinar cell carcinomas of the pancreas.
c-Ki-ras原癌基因的激活在胰腺癌中比在其他人类癌症中更为常见。大多数此类癌症为导管细胞表型。仓鼠胰腺的导管腺癌有类似的突变，但小鼠和大鼠胰腺的腺泡细胞癌缺乏常见的c-Ki-ras突变。因此，我们检测了11例人类胰腺腺泡细胞癌，以寻找第12、13和61密码子处突变的证据。从石蜡包埋切片中的肿瘤细胞中分离DNA。使用聚合酶链反应扩增适当的DNA序列，然后使用等位基因特异性寡核苷酸杂交和DNA序列分析来评估c-Ki-ras结构。在第12、13和61密码子处仅发现野生型序列。因此，在人类和啮齿类动物中，c-Ki-ras突变在导管癌发生中似乎比在胰腺腺泡细胞癌发生中更重要。
