Liu D J
Tong Ren Hospital, Beijing.
Zhonghua Er Bi Yan Hou Ke Za Zhi. 1993;28(6):358-9, 382.
We examined 16 recurrent respiratory papillomatosis patients, who were undergoing treatment for recurrence of their diseases, for the presence of human papilloma virus (HPV). Biopsies were obtained from the papillomas site of larynx or trachea. The HPV-DNA 6b, 11, 16 were determined by using Slot blot hybridization technique, type HPV-6b positive 87.5%, HPV-11 positive 93.7% HPV-16 positive 81.6%. Using Southern blot procedure examined 12 HPV-DNA positive samples, the results show that 3 were typical HPV-11.5 were undetermined types. All cases were treated by endoscopic or microendoscopic surgery repeatedly and followed up for 2-10 years. The remission rate was 71% (10/14).
我们对16例复发性呼吸道乳头状瘤病患者进行了检查，这些患者正在接受疾病复发的治疗，以检测人乳头瘤病毒（HPV）的存在。活检取自喉部或气管的乳头状瘤部位。采用斑点杂交技术检测HPV-DNA 6b、11、16型，HPV-6b阳性率为87.5%，HPV-11阳性率为93.7%，HPV-16阳性率为81.6%。采用Southern印迹法检测12份HPV-DNA阳性样本，结果显示3份为典型的HPV-11型，5份为未确定类型。所有病例均反复接受内镜或显微内镜手术治疗，并随访2至10年。缓解率为71%（10/14）。
