[Detection of human papillomavirus DNA with biotinylated probes in laryngeal papillomas by in situ hybridization].

This study used the technique of in situ hybridization (ISH) to detect human papillomavirus (HPV) 6, 11, 16 and 30 DNA genome in 45 paraffin and 3 frozen specimens of laryngeal papillomas. The results showed 17 HPV 6 and 19 HPV 11 positive in 45 paraffin specimens. The HPV 6 infection in juvenile laryngeal papillomas was significantly higher than that in adult lesions. On the other hand, there was no significant difference for HPV 11, and between male and female patients. Only one of the 3 frozen sections was positive for HPV 11 in juvenile recurrence case. HPV 16 and 30 were negative in all cases. The positive signal was always confined to the nuclei in superficial squamous epithelium of laryngeal papillomas. The results showed that the ISH technique could provide direct evidence of the association of HPV and juvenile or adult laryngeal papillomas.