Uhlig S, Wollin L
Department of Biochemical Pharmacology, University of Konstanz, Federal Republic of Germany.
J Pharmacol Toxicol Methods. 1994 Apr;31(2):85-94. doi: 10.1016/1056-8719(94)90047-7.
This article will describe a method for perfusing and ventilating rat lungs which allows assessment of lung mechanics, segmental vascular resistance, gas exchange, and vascular permeability. Isolated perfused rat lungs (IPL) were ventilated by negative pressure ventilation and were perfused at constant pressure with recirculating, albumin-containing Krebs-Henseleit buffer. A newly constructed respirator pump facilitated well-defined ventilation of the lungs and permitted switching between negative and positive pressure ventilation. Using this setup lungs were ventilated with positive pressure during both surgery and measurement of lung weight. Once the lungs were prepared, electronic control circuits ensured stability of the perfusate pH and perfusate pressure. Such preparations were stable for at least 3 hr. Breathing mechanics, for example, tidal volume, pulmonary compliance, and pulmonary resistance, perfusate characteristics, such as pulmonary vascular resistance, pulmonary pre- and postcapillary resistance, perfusate pH, PO2, and PCO2, and the capillary filtration coefficient were determined. All measured parameters were within normal physiological range, and the lungs were biochemically active, and responded to various stimuli.
本文将描述一种对大鼠肺进行灌注和通气的方法，该方法可用于评估肺力学、节段性血管阻力、气体交换和血管通透性。分离的灌注大鼠肺（IPL）通过负压通气进行通气，并在恒压下用含白蛋白的循环克氏-亨氏缓冲液进行灌注。新构建的呼吸泵有助于实现明确的肺通气，并允许在负压和正压通气之间切换。使用该装置，在手术和测量肺重量期间，肺均采用正压通气。一旦肺制备完成，电子控制电路可确保灌注液pH值和灌注液压力的稳定。此类制备物至少可稳定3小时。测定了呼吸力学参数，如潮气量、肺顺应性和肺阻力；灌注液特性，如肺血管阻力、肺毛细血管前和后阻力、灌注液pH值、PO2和PCO2；以及毛细血管滤过系数。所有测量参数均在正常生理范围内，肺具有生化活性，并对各种刺激有反应。
