Bienz K, Egger D, Pfister T
Institute for Medical Microbiology, University of Basel, Switzerland.
Arch Virol Suppl. 1994;9:147-57. doi: 10.1007/978-3-7091-9326-6_15.
In the infected cell, the poliovirus replication complex (RC) is found in the center of a rosette formed by many virus-induced vesicles. The RC is attached to the vesicular membranes and contains a compact central part which encloses the replication forks of the replicative intermediate and all proteins necessary for strand elongation. The growing plus strands of the replicative intermediate protrude from the central part of the RC, but are still enclosed by membraneous structures of the rosette. After completion, progeny 36S RNA is set free at the surface of the rosette. In an in vitro transcription system, isolated replication complex-containing rosettes are active in initiation, elongation and maturation (release) of plus strand progeny RNA. Full functionality of the RC depends on an intact structural framework of all membraneous components of the rosette.
在受感染的细胞中，脊髓灰质炎病毒复制复合体（RC）位于由许多病毒诱导的囊泡形成的玫瑰花结中心。RC附着于囊泡膜，包含一个紧密的中央部分，该部分包围复制中间体的复制叉以及链延伸所需的所有蛋白质。复制中间体不断增长的正链从RC的中央部分伸出，但仍被玫瑰花结的膜结构所包围。完成后，子代36S RNA在玫瑰花结表面释放。在体外转录系统中，分离出的含有复制复合体的玫瑰花结在正链子代RNA的起始、延伸和成熟（释放）过程中具有活性。RC的完整功能取决于玫瑰花结所有膜成分的完整结构框架。
