Murtaugh M P, Elam M R, Kakach L T
Department of Veterinary PathoBiology, University of Minnesota, St. Paul, USA.
Arch Virol. 1995;140(8):1451-60. doi: 10.1007/BF01322671.
The 3'-portion of the genome of a U.S. isolate of the porcine reproductive and respiratory syndrome (PRRS) virus, ATCCVR-2332, was cloned and sequenced. The resultant 3358 nucleotides contain 6 open reading frames (ORFs) with homologies to ORFs 2 through 7 of the European strain of the PRRS virus and other members of the free-standing genus of arteriviruses. Both VR-2332 and the European isolate (called the Lelystad virus) have been identified as infectious agents responsible for the swine disease called PRRS. Comparative sequence analysis indicates that there are degrees of amino acid identity to the Lelystad virus open reading frames ranging from 55% in ORF 5 to 79% in ORF 6. Hydropathy profiles indicate that the ORFs of VR-2332 and Lelystad virus correspond structurally despite significant sequence differences. These results are consistent with the biological similarities but distinct serological properties of North American and European isolates of the virus.
对一株美国猪繁殖与呼吸综合征（PRRS）病毒（ATCC VR - 2332）基因组的3'部分进行了克隆和测序。所得的3358个核苷酸包含6个开放阅读框（ORF），与PRRS病毒欧洲株的ORF 2至7以及动脉炎病毒属其他成员具有同源性。VR - 2332和欧洲分离株（称为莱利斯塔德病毒）均已被确定为导致猪病PRRS的感染因子。比较序列分析表明，与莱利斯塔德病毒开放阅读框的氨基酸同一性程度在ORF 5中为55%，在ORF 6中为79%。亲水性图谱表明，尽管序列存在显著差异，但VR - 2332和莱利斯塔德病毒的ORF在结构上相对应。这些结果与该病毒北美和欧洲分离株的生物学相似性但血清学特性不同是一致的。
