Slootweg P J, Koole R, Hordijk G J
Department of Pathology, University Hospital, Utrecht, The Netherlands.
Eur J Cancer B Oral Oncol. 1994;30B(2):138-41. doi: 10.1016/0964-1955(94)90066-3.
Paraffin embedded material from 15 patients suffering from head and neck squamous cell carcinoma (HNSCC) bordered by dysplastic mucosal areas was immunohistochemically investigated for the presence of p53 protein and Ki-67 proliferation marker. p53 protein was present in 9 cases (60%), invariably in invasive cancer areas as well as in adjacent non-invasive dysplastic mucosa. Only cells exhibiting atypia contained p53 protein. Ki-67 proliferation marker was present in the basal cells of the normal epithelium and more extensive in dysplasias and HNSCC. The presence of Ki-67 closely coincided with p53 protein in the 9 cases exhibiting this. No differences in Ki-67 expression were found between p53 positive and negative cases. It is concluded that the appearance of p53 protein occurs early in carcinogenesis but that cells also may show increased proliferation without involving immunohistochemically detectable alterations in the p53 gene.
对15例头颈部鳞状细胞癌（HNSCC）且伴有发育异常黏膜区域的石蜡包埋材料进行免疫组织化学研究，以检测p53蛋白和Ki-67增殖标志物的存在情况。9例（60%）存在p53蛋白，均见于浸润癌区域以及相邻的非浸润性发育异常黏膜。只有表现出异型性的细胞含有p53蛋白。Ki-67增殖标志物存在于正常上皮的基底细胞中，在发育异常和HNSCC中更为广泛。在9例出现这种情况的病例中，Ki-67的存在与p53蛋白密切相关。p53阳性和阴性病例之间未发现Ki-67表达的差异。得出的结论是，p53蛋白在致癌过程中早期出现，但细胞也可能表现出增殖增加，而p53基因在免疫组织化学上未检测到改变。
