用于测定人血清中硝苯地平的色谱方法评估。

Evaluation of chromatographic methods for the determination of nifedipine in human serum.

作者信息

Jankowski A, Lamparczyk H

机构信息

Department of Biopharmaceutics, Medical Centre of Postgraduate Education, Bydgoszcz, Poland.

出版信息

J Chromatogr A. 1994 May 13;668(2):469-73. doi: 10.1016/0021-9673(94)80141-X.

DOI:10.1016/0021-9673(94)80141-X

PMID:8032493

Abstract

Gas-liquid chromatography and high-performance liquid chromatography were compared for the identification and determination of nifedipine in biological samples and the elaboration of the optimum liquid-liquid extraction procedure. The determination limits were 2 and 10 ng/ml, respectively, and the detection limits were 1 and 5 ng/ml, respectively. The calibration graphs were linear in the ranges 2-300 and 10-500 ng/ml, respectively. Recoveries based on three different concentrations were 88.7-95.8% and 93.7-104.2%, respectively. Both methods are sensitive, specific and reproducible enough for pharmacokinetic studies and therapeutic drug monitoring.

摘要

比较了气相色谱法和高效液相色谱法用于生物样品中硝苯地平的鉴定和测定以及优化液-液萃取程序。测定限分别为2和10 ng/ml，检测限分别为1和5 ng/ml。校准曲线分别在2 - 300和10 - 500 ng/ml范围内呈线性。基于三种不同浓度的回收率分别为88.7 - 95.8%和93.7 - 104.2%。两种方法对于药代动力学研究和治疗药物监测都足够灵敏、特异且可重复。