[乳酸脱氢酶、琥珀酸脱氢酶检测冷冻对MEC-1、Ca9-22、Tca8113细胞系的体外影响]

[LDH, SDH assay for the effect of freezing on MEC-1, Ca9-22, Tca8113 cell lines in vitro].

作者信息

Wang W

机构信息

Stomatological Hospital, Fourth Millitary Medical University, Xian.

出版信息

Zhonghua Kou Qiang Yi Xue Za Zhi. 1993 Nov;28(6):359-61, 384.

PMID:8033648

Abstract

Three human oral cancer cell lines were tested with the purpose of investigating the effect of freezing temperature and freeze-thaw cycle on the cells by LDH-release, SDH assay. The results showed that freezing can kill the cancer cells. The effective freezing temperature for cryonecrosis of the cells was -40 degrees C-(-)50 degrees C. Two freeze-thaw cycles were potent enough to obtain the most effectual cryonecrosis of the cancer cell lines. Amount of LDH-release was freezing-temperature dependent. At -40 degrees C, more then 90% LDH of cells were released. The results showed that the membranes of 90% survival cells were injured after cryotreat.

摘要

为了通过乳酸脱氢酶（LDH）释放法和琥珀酸脱氢酶（SDH）测定法研究冷冻温度和冻融循环对细胞的影响，对三种人类口腔癌细胞系进行了测试。结果表明，冷冻可杀死癌细胞。细胞冷冻坏死的有效冷冻温度为-40℃至-50℃。两个冻融循环足以使癌细胞系获得最有效的冷冻坏死效果。LDH释放量取决于冷冻温度。在-40℃时，超过90%的细胞LDH被释放。结果表明，冷冻处理后90%存活细胞的细胞膜受到损伤。