Storm H, Rognum T O, Reichelt K L
Department of Paediatric Research, Rikshospitalet, Oslo, Norway.
Eur J Pediatr. 1994 May;153(5):381-6. doi: 10.1007/BF01956426.
In nucleus tractus solitarius (NTS) beta-endorphin (BEND) induces bradycardia and respiratory depression which have been reported to precede death in sudden infant death (SID). Of SID victims, 50% have elevated levels of beta-endorphin immunoreactivity (BENDI) in the cerebrospinal fluid (CSF), and 50% had undetectable levels. We therefore investigated the relationship of BENDI in the CSF to BENDI levels in the NTS area. This study included SID victims (CSF from n = 47, brain stem from n = 16), borderline SID victims (CSF and brain stem from n = 2), sudden death in childhood (CSF and brain stem from n = 1), and controls (CSF from n = 32, brain stem from n = 11). BEND in CSF and NTS area, after extraction, was measured by radioimmunoassay. High performance liquid chromatography was used for closer identification of BENDI. We found that the SID victims divided into two subpopulations, one having a relatively high BENDI level in CSF and one having no detectable level (P < 0.01). Furthermore, an inverse relationship was found between BENDI level in CSF and BENDI level in NTS area in the SID victims (P < 0.05). We conclude that increased BENDI level in CSF is associated with low BENDI level in the NTS area in 50% of SID victims. The low BENDI level in the NTS area may be due to increased release of BEND.
在孤束核（NTS）中，β-内啡肽（BEND）可诱发心动过缓和呼吸抑制，据报道，这两种情况在婴儿猝死（SID）中先于死亡出现。在SID受害者中，50%的脑脊液（CSF）中β-内啡肽免疫反应性（BENDI）水平升高，50%的检测不到。因此，我们研究了CSF中的BENDI与NTS区域中BENDI水平之间的关系。本研究包括SID受害者（47例的CSF，16例的脑干）、临界SID受害者（2例的CSF和脑干）、儿童期猝死（1例的CSF和脑干）以及对照组（32例的CSF，11例的脑干）。提取后，通过放射免疫分析法测量CSF和NTS区域中的BEND。使用高效液相色谱法对BENDI进行更精确的鉴定。我们发现，SID受害者分为两个亚组，一组CSF中的BENDI水平相对较高，另一组检测不到（P<0.01）。此外，在SID受害者中，发现CSF中的BENDI水平与NTS区域中的BENDI水平呈负相关（P<0.05）。我们得出结论，在50%的SID受害者中，CSF中BENDI水平升高与NTS区域中BENDI水平低有关。NTS区域中BENDI水平低可能是由于BEND释放增加所致。
