Macciò A, Mantovani G, Turnu E, Artini P, Contu G, Volpe A
Clinica Ostetrica e Ginecologica, Università degli Studi di Cagliari, Ospedale San Giovanni di Dio, Italy.
Fertil Steril. 1994 Aug;62(2):327-32. doi: 10.1016/s0015-0282(16)56886-1.
To evaluate the immunoactivity of human follicular fluids (FFs) of ovaries stimulated with exogenous gonadotropins.
Follicular fluids and peripheral blood mononuclear cells (PBMCs) were obtained at the time of ovum pick-up from 15 women (25 to 34 years of age) undergoing gonadotropin stimulation for GIFT.
The evaluation of the blastic response of PBMC to phytohemagglutinin (PHA), PHA plus recombinant interleukin (IL)-2, and recombinant IL-2 alone was performed by measuring 3H-thymidine (3H-TdR) incorporation by the cells cultured in media with or without FF. The percentage of CD25 expression on PHA-stimulated T lymphocytes was evaluated by flow cytofluorometry. The levels of IL-1 alpha and IL-2 in supernatants from PHA-stimulated PBMC were evaluated by an ELISA.
The PBMC blastic response to PHA, to recombinant IL-2 alone, and to PHA plus recombinant IL-2 was suppressed by the addition of FF. However, the percentage of inhibition of 3H-TdR incorporation induced by FF in cultures performed in the presence of PHA alone was significantly higher than that obtained in cultures performed in the presence of PHA plus recombinant IL-2. The percentage of expression of the CD25 on PHA-stimulated lymphocytes was also significantly lower in presence of FF in comparison with the controls. Similarly, the production of IL-1 alpha and IL-2 in culture media of PHA-stimulated PBMC was significantly lower when FF was added in culture medium.
The present data confirm that preovulatory FFs obtained by gonadotropin-stimulated ovaries contain unknown factors that induce immunosuppression. Furthermore, our study explains that the immunosuppressive activity of FF is mediated by the specific inhibition of the production of IL-1 alpha and IL-2 and by the decreased expression of the CD25.
评估外源性促性腺激素刺激后人类卵泡液（FF）的免疫活性。
在取卵时从15名接受促性腺激素刺激以进行配子输卵管内移植（GIFT）的女性（25至34岁）获取卵泡液和外周血单个核细胞（PBMC）。
通过测量在添加或不添加FF的培养基中培养的细胞对3H - 胸腺嘧啶核苷（3H - TdR）的掺入，来评估PBMC对植物血凝素（PHA）、PHA加重组白细胞介素（IL）-2以及单独重组IL - 2的增殖反应。通过流式细胞荧光术评估PHA刺激的T淋巴细胞上CD25的表达百分比。通过酶联免疫吸附测定法（ELISA）评估PHA刺激的PBMC上清液中IL - 1α和IL - 2的水平。
添加FF可抑制PBMC对PHA、单独重组IL - 2以及PHA加重组IL - 2的增殖反应。然而，在仅存在PHA的培养物中，FF诱导的3H - TdR掺入抑制百分比显著高于在存在PHA加重组IL - 2的培养物中获得的抑制百分比。与对照组相比，在存在FF的情况下，PHA刺激的淋巴细胞上CD25的表达百分比也显著降低。同样，当在培养基中添加FF时，PHA刺激的PBMC培养基中IL - 1α和IL - 2的产生也显著降低。
目前的数据证实，促性腺激素刺激卵巢获得的排卵前FF含有诱导免疫抑制的未知因子。此外,我们的研究表明，FF的免疫抑制活性是通过特异性抑制IL - 1α和IL - 2的产生以及CD25表达的降低来介导的。
